No statistical methods were used to predetermine sample size. The experiments were not randomized, and investigators were not blinded to allocation during experiments and outcome assessment.
Cell line sources
CHM13hTERT (CHM13) cells were originally isolated from a hydatidiform mole at Magee-Womens Hospital as part of a research study (IRB MWH-20-054). Cryogenically frozen cells from this culture were grown and transformed with the human telomerase reverse transcriptase (TERT) gene to immortalize the cell line. This cell line has been authenticated by STR analysis, tested negative for mycoplasma contamination, and karyotyped to show a 46,XX karyotype13. Human HG00733 lymphoblastoid cells were originally obtained from a female Puerto Rican child, immortalized with the Epstein-Barr virus (EBV), and stored at the Coriell Institute for Medical Research. Chimpanzee (Pan troglodytes; Clint; S006007) fibroblast cells were originally obtained from a male western chimpanzee named Clint (now deceased) at the Yerkes National Primate Research Center and immortalized with EBV. Orangutan (Pongo abelii; Susie; PR01109) fibroblast cells were originally obtained from a female Sumatran orangutan named Susie (now deceased) at the Gladys Porter Zoo, immortalized with EBV, and stored at the Coriell Institute for Medical Research. Macaque (Macaca mulatta; AG07107) fibroblast cells were originally obtained from a female rhesus macaque of Indian origin and stored at the Coriell Institute for Medical Research. The HG00733, chimpanzee, orangutan